CHARACTERIZATION OF DIFFERENTIAL EXPRESSION PATTERNS OF THE EXTRACELLULAR PURINERGIC ENZYMES IN COLORECTAL CANCER

The aim of this study is to characterize tumor cell specific expression purinergic ecto-enzymes CD39 and CD73, associate prognostic significance these patterns in colorectal cancer (CRC) patients. Protein gene the target genes various CRC lines were assessed via Western Blot (WB) analysis Real Time PCR (RT-PCR). Additionally, vs stromal was analyzed from publicly available patient datasets. Finally, correlation between CD73 with prognosis Cancer Genome Atlas (TCGA) In lines, found be not expressed at all while extensively most WB RT-PCR analyses. Patient microarray data confirmed results that very low epithelial/tumor cells relative other types yet abundantly every type within samples. Interestingly, tumors correlated favorable associated worse prognosis. Although are related enzymes involved extracellular signaling, their effects patients show opposing outcomes. Therefore, better insights understanding functional involvement development needed further mechanistic studies.